Primer concentration and Pre-denaturation Time Effect on <i>cyt-K Bacillus cereus</i> Detection using Real-Time PCR Method

Rapid Detection of COVID-19 by RT-LAMP PCR Technique and its Comparison with Real-Time RT-PCR Method

Rapid antigen and antibody, serological tests, and RT ‑ PCR-based molecular methods are widely used for the detection of microorganisms worldwide. Objectives: This study aimed by Isothermal nucleic acid amplification techniques to detect the covid-19. Methods: In this study, 200 samples of nasopharynx and oropharynx were collected from Jamaran Heart Hospital in Tehran. Covid -19 was exami...

Real-time damage detection of bridges using adaptive time-frequency analysis and ANN

Although traditional signal-based structural health monitoring algorithms have been successfully employed for small structures, their application for large and complex bridges has been challenging due to non-stationary signal characteristics with a high level of noise. In this paper, a promising damage detection algorithm is proposed by incorporation of adaptive signal processing and Artificial...

Coamplification at lower denaturation temperature-PCR increases mutation-detection selectivity of TaqMan-based real-time PCR.

BACKGROUND DNA genotyping with mutation-specific TaqMan(R) probes (Applied Biosystems) is broadly used in detection of single-nucleotide polymorphisms but is less so for somatic mutations because of its limited selectivity for low-level mutations. We recently described coamplification at lower denaturation temperature-PCR (COLD-PCR), a method that amplifies minority alleles selectively from mix...

Real-time PCR detection chemistry.

Real-time PCR is the method of choice in many laboratories for diagnostic and food applications. This technology merges the polymerase chain reaction chemistry with the use of fluorescent reporter molecules in order to monitor the production of amplification products during each cycle of the PCR reaction. Thus, the combination of excellent sensitivity and specificity, reproducible data, low con...

Detection of Congenital Mullerian Anomalies Using Real-Time 3D Sonography